9/11/2023 0 Comments 10x chromium gem![]() Pair heavy and light chain immunoglobulin sequences from individual B-cells.Pair α and β chain TCR sequences from individual T-cells.Characterize individual T-cells and B-cells.Reveal immune cell clonality, diversity, antigen specificity, and cellular context.Just like the Single Cell Gene Expression solution, you can do this for 3,000 – 10,000 cells per sample. The solution allows for analyzing both the T-cell and B-cell receptor. The Single Cell Immune Profiling solution provides you with data on the immune repertoire and gene expression. Read more on the website of 10x Genomics Single Cell Immune Profiling Sometimes this solution is not the best one for your project. Compare sample before and after treatment.Determine heterogeneity of your sample.The Single Cell Gene Expression solution provides single-cell transcriptomics data. It allows you to measure the 3’ gene expression for 3,000 –10,000 cells per sample. This high-throughput solution has a cost-effective price per cell.Īpplications of this solution are for example: For both solutions, we are a Certified Service Provider. Whatever species or tissue you are working on, you are probably not the first to use 10x Genomics.Ĭurrently, we offer two solutions of 10x Genomics: Single Cell Gene Expression and Single Cell Immune Profiling. The platform has currently been used in more than 1,000 publications, so you are far from alone. It’s the leading microfluidics platform.Combining products allows for obtaining single-cell data with multiple dimensions. This results in a low cost per cell in the case of high-throughput projects.ġ0x Genomics offers multiple products, of which some can be combined. The 10x Genomics systems allows for targeting of thousands of cells per sample. After sequencing, reads are mapped back to the corresponding single cell.Barcoded fragments are pooled for downstream reactions to create sequencing libraries.Within the GEM, a reaction takes place where GEL beads are dissolved and molecules from a single cell are captured and barcoded. ![]() Within the Chromium controller, barcoded Gel Beads are mixed with cells or nuclei, enzymes, and partitioning oil to form “GEMs” (Gel Bead-in-emulsion), which are single-cell emulsion droplets.A pool of Gel Beads, coated with barcoded primers, cells and enzymes are loaded on the 10x Genomics microfluidics chip and placed in the chromium controller.This how the Next GEM technology in the Chromium controller works: Currently, we offer Single Cell Gene Expression and Single Cell Immune Profiling. The biology behind 10x Genomicsġ0x Genomics offers multiple products that can be used on the chromium controller, each with their own applications. The technique makes use of the Chromium system, a device that enables single-cell sequencing with their Next GEM technology. Getting started In-depth knowledge and support helpġ0x Genomics is a microfluidics-based method of single-cell RNA sequencing. ![]()
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